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BACKGROUND: Transfusion-transmissible hepatitis B virus (HBV) infection is a major problem worldwide. Recently, confirmatory nucleic acid tests (NATs) for HBV DNA have been employed in several countries. We assessed the prevalence and yearly trends of HBV infection in blood donors in the Eastern Province of Saudi Arabia, screening for HBV surface antigen (HBsAg), antibody against HBV core antigen (anti-HBc), and HBV DNA. METHODS: Between 2011 and 2015, a total of 22,842 donors were screenedfor HBsAg, anti-HBc, and HBV DNA using the HBsAg Qualitative II kit (Abbott, Ireland Diagnostics Division, Sligo, Ireland), ARCHITECT Anti-hepatitis B core antigen antibody (HBc) II Assay kit (Abbott GmbH & Co. KG, Wiesbaden, Germany), and NAT Procleix Ultrio Elite Assay kit (Grifols Diagnostic Solutions Inc., Los Angeles, CA, USA), respectively. RESULTS: A total of 739 (3.24%) donors were HbsAg(+), anti-HBc(+), or HBV DNA(+); 63 (0.28%) were HbsAg(+), anti-HBc(+), and HBV DNA(+). Twelve (0.05%) were anti-HBc(+) and HBV DNA(+) but HBsAg(−); they were considered to have occult infection. Further, 664 (2.91%) were HBsAg(−) but anti-HBc(+), indicating chronic or resolving infection. HBV prevalence increased significantly from 2011 to 2012, increased marginally till 2013, and showed a decreasing trend from 2013 (P>0.05). CONCLUSIONS: The five-year prevalence of HBV infection among blood donors in the Eastern Province of Saudi Arabia (3.24%) is lower than that reported for other regions in the country. The occult HBV infection rate of 0.05% emphasizes the importance of NATs in isolating potential infectious blood units.
Subject(s)
Humans , Antigens, Surface , Blood Donors , DNA , Hepatitis B Surface Antigens , Hepatitis B virus , Hepatitis B , Hepatitis , Ireland , Mass Screening , Prevalence , Retrospective Studies , Saudi Arabia , Tissue DonorsABSTRACT
Aims@#Vibrio parahaemolyticus is a marine and estuarine bacterium that has been documented as the causative agent of food-borne outbreak worldwide. The aim of this study was to confirm the identification of presumptive V. parahaemolyticus isolates to the species level by using PCR targeted to the outer membrane protein regulation operon gene (toxR) and to investigate antibiotic resistance, plasmid profile, and the main core virulence genes of thermostable direct hemolysin (tdh) and tdh-related hemolysin (trh). @*Methodology and results@# A total of 56 presumptive isolates of V. parahaemolyticus were isolated from seawater collected during year a 2010 sampling pilot study performed along the Arabian Gulf coast of the Eastern Province of Saudi Arabia. The purpose of this study was to confirm the identification of presumptive V. parahaemolyticus isolates to the species level by using PCR targeted to the toxR gene and to investigate antibiotic resistance, plasmid profile, and the main core virulence genes of tdh and trh. The toxR-specific PCR assay revealed that a total of 30 out of 56 isolates tested positive for V. parahaemolyticus. None of the 30 strains of the toxR gene were tested positive for tdh and trh genes. All (100%) of isolates were highly resistant to amikacin, cefuroxime, ampicillin, ticarcillin, cefaclor (80%), and tetracycline (70%). The multiple antibiotic resistance (MAR) index was measured for all 16 antimicrobial agents, and the high ranged from 0.25 to 0.56. Among the isolated V. parahaemolyticus, 22 out of 30 strains contained plasmid DNA bands ranging in size from 1.5 to 55 kb and no correlation was observed between the plasmid profiles and antibiotic resistance patterns. @*Conclusion, significance and impact of study@#The results obtained in this study indicate that V. parahaemolyticus is present in the coastal environment of the Eastern Province of Saudi Arabia.
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Background: the prevalence of extended-spectrum [beta]-lactamase [ESBL] production and antimicrobial susceptibility testing in the Escherichia coli in frozen freshwater fish imported into Saudi Arabia have not been investigated
Objective: the aim of this study was to investigate the prevalence of ESBL-producing E. coli in frozen freshwater fish imported into Saudi Arabia and retailed in various supermarkets and food stores in the Eastern Province of Saudi Arabia
Materials and Methods: a total of 405 imported freshwater fish samples: Catfish [n = 65]; mrigal [n = 45]; tilapia [n = 135]; carfoo [n = 50]; rohu [n = 75]; and milkfish [n = 35] were purchased from supermarkets and screened for ESBL-producing E. coli using ESBL chromogenic selective agar. The phenotypically confirmed ESBL isolates were further tested for antimicrobial susceptibility testing against 21 antimicrobial agents and amplification of bla[TEM], bla[SHV], and bla[CTX-M] genes using polymerase chain reaction
Results: a total of 110 out of the 405 [27.2%] freshwater fish samples were found to be positive for ESBL producing E. coli and yielded 224 confirmed isolates. The highest rates of multi-drug resistant patterns to antimicrobial agents were observed in E. coli isolated from catfish, mrigal, and tilapia imported from Thailand and milkfish imported from Vietnam. The most prevalent ESBL gene found in the samples was bla[CTX-M], which was detected in tilapia [100%, n = 30] imported from Thailand and carfoo [100%, n = 5], milkfish [60%, n = 24], catfish [52.3%, n = 34], and tilapia imported from India [34.8%, n = 24]
Conclusion: the results confirmed the imported frozen freshwater fish is pool reservoir of antibiotic resistance and ESBL producing E. coli
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Objective:To determine the proportion of imported frozen fish contaminated with Salmonella among retail food stores and supermarkets in the Eastern Province of Saudi Arabia. Methods: A total of 223 frozen freshwater fish purchased from different supermarkets and grocery stores were analyzed for the presence of foodborne pathogen Salmonella. The isolation of Salmonella was determined and confirmed by using the methods of US Food and Drug Administration’s Bacteriological Analytical Manual, CHROMagar Salmonella plus, biochemical tests and API 20E strips. Antimicrobial susceptibilities of Salmonella isolates were determined by the disk diffusion method on Muller-Hinton agar, as described by Kirby-Bauer, in accordance with the guidelines of the Clinical and Laboratory Standards Institute. Results:Out of the total 223 fish samples (20 of catfish, 18 of carfu, 20 of mirgal, 25 of milkfish, 35 of mackerel, 75 of tilapia, and 30 of rohu), 89 (39.9%) were tested positive for Salmonella. The prevalence of positive samples were reported for the freshwater fish of pangas (60.0%, n=12), carfu (27.7%, n=5), mirgal (35.0%, n=7), milkfish (52.0%, n=13), mackerel (31.4 %, n=11), tilapia imported from Thailand (64.0%, n=16), tilapia imported from India (28.0%, n=14), rohu imported from Thailand (26.6%, n=4) and rohu imported from Myanmar (46.6%, n=7). A total of 140 isolates of Salmonella spp. were yielded from at least seven different types of frozen freshwater fish imported from 5 different countries and were tested for their susceptibility to 16 selected antimicrobial agents. The highest antibiotic resistance was observed to tetracycline (90.71%) followed by ampicillin (70%) and amoxicillin-clavulanic acid (45%). Conclusions: The obtained results of this study shows that these raw retail imported frozen freshwater fish are contaminated with potentially pathogenic Salmonella spp. And the study recommend and suggest that there is a need for adequate consumer measures.
ABSTRACT
<p><b>OBJECTIVE</b>To determine the proportion of imported frozen fish contaminated with Salmonella among retail food stores and supermarkets in the Eastern Province of Saudi Arabia.</p><p><b>METHODS</b>A total of 223 frozen freshwater fish purchased from different supermarkets and grocery stores were analyzed for the presence of foodborne pathogen Salmonella. The isolation of Salmonella was determined and confirmed by using the methods of US Food and Drug Administration's Bacteriological Analytical Manual, CHROMagar Salmonella plus, biochemical tests and API 20E strips. Antimicrobial susceptibilities of Salmonella isolates were determined by the disk diffusion method on Muller-Hinton agar, as described by Kirby-Bauer, in accordance with the guidelines of the Clinical and Laboratory Standards Institute.</p><p><b>RESULTS</b>Out of the total 223 fish samples (20 of catfish, 18 of carfu, 20 of mirgal, 25 of milkfish, 35 of mackerel, 75 of tilapia, and 30 of rohu), 89 (39.9%) were tested positive for Salmonella. The prevalence of positive samples were reported for the freshwater fish of pangas (60.0%, n=12), carfu (27.7%, n=5), mirgal (35.0%, n=7), milkfish (52.0%, n=13), mackerel (31.4 %, n=11), tilapia imported from Thailand (64.0%, n=16), tilapia imported from India (28.0%, n=14), rohu imported from Thailand (26.6%, n=4) and rohu imported from Myanmar (46.6%, n=7). A total of 140 isolates of Salmonella spp. were yielded from at least seven different types of frozen freshwater fish imported from 5 different countries and were tested for their susceptibility to 16 selected antimicrobial agents. The highest antibiotic resistance was observed to tetracycline (90.71%) followed by ampicillin (70%) and amoxicillin-clavulanic acid (45%).</p><p><b>CONCLUSIONS</b>The obtained results of this study shows that these raw retail imported frozen freshwater fish are contaminated with potentially pathogenic Salmonella spp. And the study recommend and suggest that there is a need for adequate consumer measures.</p>